Derivatives of bead cellulose:
Reactive Sorbents:
Selected applications:
More informations:
Macroporous Bead Cellulose MT
Gel filtration media for biomolecule separations | General informations
On this page:
- ↓ Features.
- ↓ Mechanical stabiliy.
- ↓ Total pore volume.
- ↓ Size exclusion limits.
- ↓ Other technical data.
Macroporous bead cellulose has a hydrophilic matrix and spherical particles. It is produced in several porosity types, marked MT 50, MT 100, MT 200, MT 500 and various particle size. Macroporous Bead Cellulose MT is a highly porous regenerated cellulose with heterogenous matrix (partially microcrystalline) and with polymer structure stabilized by hydrogen bonds only - there are no covalent cross-linkings.
By their pressure stability Macroporous Bead Cellulose MT belongs to the category of swollen semi-rigid gels for low-pressure chromatography. The gels are characterized by excellent mechanical properties.
Features
- Excellent mechanical stability even at gel with large pores. Easy to handle. Resistant to destruction and generation of fine particles during stirring.
- Rigid spherical particles. High flow throughput the gels at a low drop in pressure.
- Narrow particle size distribution in several degrees converting all ranges common for this sort of gel.
- High chemical resistance and compatibility with most commonly used solvents and buffers. Applicability in wide range of pH and salt concentrations with minimal changes (swelling / shrinking) in the gel bed.
- High temperature stability allows sterilization by autoclaving.
- High hydrophility. A number of free hydroxy groups in matrix allows preparation of derivatives or enzyme immobilization.
- High porosity of matrix allows good capacity and recovery.
- High selectivity of separation.
- Easy-to-ready. Gels are supplied pre-swollen.
Mechanical stability
Values of pure Bead Cellulose only (non-magnetic and non-derivated)
| Types | Click on image for enlarge a graph. | Particle Size [μm] | Flow Performance for destiled water at 20°C [ml cm-2 hod-1 bar-1 m-1] |
|---|---|---|---|
MT 50 |
 | 30 - 50 | 85 |
| 50 - 100 | 270 | ||
| 100 - 250 | > 500 | ||
MT 100 |  | 30 - 50 | 20 |
| 50 - 80 | 170 | ||
| 80 - 100 | 300 | ||
| 100 - 250 | 600 | ||
MT 200 |
 | 50 - 80 | 130 |
| 80 - 100 | 220 | ||
| 100 - 250 | 450 | ||
MT 500 |  | 50 - 80 | 45 |
| 80 - 100 | 80 | ||
| 100 - 250 | 250 |
| Types | Click on image for enlarge a graph | Particle size [µm] | Other description |
|---|---|---|---|
| MT 100, MT 200, MT 500 |  | 50 - 100 | Gel bed 1x60 cm |
| MT 100, 200, 500, Agarose gel, Japan Cellulose 2000. |  | 50 - 100 | Column: 10 x 660 mm; Eluent: buffer phosphate 0.2 M, pH = 7; Packing Pressure: 1 bar |
| MT 50, Japan Cellulose 90, 300, 1000, Dextran gel 1000 |
 | 50 - 100 | Column: 10 x 660 mm Eluent: destilled water |
| Types | Pressure [bar] |
|---|---|
| MT 50 | > 2.0 |
| MT 100 | 1.5 - 2.0 |
| MT 200 | 1.2 - 1.5 |
| MT 500 | 0.8 - 1.2 |
| Types | Flow Rate | Pressure [bar] | |
|---|---|---|---|
| linear [cm hr-1] | volumetric for bead size range 50-80 µm and gel-bed 1x60 cm [ml min-1] | ||
| MT 50 | > 760 | > 9.9 | > 4.0 |
| MT 100 | 750 | 9.0 | 2.2 - 2.7 |
| MT 200 | 450 | 6.0 | 1.5 - 2.0 |
| MT 500 | 115 | 1.5 | 1.0 - 1.5 |
Total pore volume
Values of pure Bead Cellulose only (non-magnetic and non-derivated)
| The pore volume determined as W.R. | ||
|---|---|---|
| Porozity | Total Pore Volume Dry [cm3 g-1] |
Total Pore Volume Sucked [cm3 g-1] |
| MT 50 | 1.3 | 0.57 |
| MT 100 | 5.8 | 0.85 |
| 200 | 8.0 | 0.89 |
| 300 | 9.2 | 0.90 |
| 500 | 11.5 | 0.92 |
| Average values of Total Pore Volume for various porozities of Macroporous Bead Cellulose MT |
 |
| Types | Net volume of water-swollen beads [cm3 g-1] Does not include the interstitial spaces. | Medium pore diameter [nm] |
|---|---|---|
| MT 50 | 1.65 | 7.4 |
| MT 100 | 4.86 | 27 |
| MT 200 | 6.71 | 32 |
| MT 300 | 7.32 | 38 |
| MT 500 | 7.43 | 38 |
These resutls in this table were measured by:
Ing. Karel Jeřábek, Ph.D.
Institute of Chemical Process Fundamentals
Rozvojová 135
165 02 Prague 6
Czech Republic
Size exclusion limits
Values of pure Bead Cellulose only (non-magnetic and non-derivated)
Macroporous Bead Cellulose MT types are highly porous gels well accessible for large macromolecules, as it is shown in these Figures.
| Selectivity curves of Macroporous Bead Cellulose MT types Kav vs. Molecular Weight of globular proteins in semilogarithmic scale |
 |
| Protein | M. W. | Kav | |||
|---|---|---|---|---|---|
| [g mol-1] | MT 50 | MT 100 | MT 200 | MT 500 | |
| Blue Dextran | 2 000 000 | 0 | 0 | 0.01 | 0.02 |
| IgM | 1 000 000 | 0 | 0.10 | 0.22 | 0.36 |
| Thyroglobulin | 660 000 | 0.01 | 0.20 | 0.40 | 0.50 |
| Ferritin Eluent: buffer 0.013 M phosphate pH 7.0 | 440 000 | 0.01 | 0.35 | 0.57 | 0.65 |
| Catalase Eluent: buffer 0.013 M phosphate pH 7.0 | 238 000 | 0.01 | 0.47 | 0.74 | 0.78 |
| Aldolase | 158 000 | 0.02 | 0.55 | 0.78 | 0.81 |
| GLS | 135 000 | 0.05 | 0.58 | 0.80 | 0.82 |
| Bovine Serum Albumin | 66 000 | 0.19 | 0.70 | 0.88 | 0.87 |
| Ovalbumin | 44 000 | 0.30 | 0.75 | 0.88 | 0.88 |
| Chymotrypsinogen A | 25 000 | 0.44 | 0.81 | 0.89 | 0.90 |
| Myoglobulin | 18 000 | 0.50 | 0.83 | 0.90 | 0.91 |
| Ribonuclease | 13 500 | 0.55 | 0.85 | 0.90 | 0.92 |
| Cytochrome C | 12 400 | 0.57 | 0.86 | 0.91 | 0.92 |
| Bacitracin | 1 420 | 0.71 | 0.89 | 0.92 | 0.95 |
| L-Histidin | 152 | 0.72 | 0.90 | 0.93 | 0.96 |
| Alanine | 89 | 0.73 | 0.91 | 0.94 | 0.97 |
| Selectivity curves of Macroporous Bead Cellulose MT types Kav vs. Molecular Weight of Dextran in semilogarithmic scale |
 |
| DEXTRAN Molecular Weight | Kav | ||||
|---|---|---|---|---|---|
| MT 50 | MT 100 | MT 200 | MT 300 | MT 500 | |
| 1 500 | 0.45 | 0.94 | 0.93 | 0.99 | 0.99 |
| 6 000 | 0.18 | 0.79 | 0.81 | 0.98 | 0.98 |
| 10 000 | 0.01 | 0.72 | 0.75 | 0.84 | 0.83 |
| 17 500 | 0 | 0.62 | 0.67 | 0.76 | 0.76 |
| 40 000 | 0 | 0.45 | 0.52 | 0.63 | 0.62 |
| 70 000 | 0 | 0.31 | 0.39 | 0.51 | 0.50 |
| 110 000 | 0 | 0.20 | 0.28 | 0.41 | 0.39 |
| 196 300 | 0 | 0.07 | 0.15 | 0.26 | 0.25 |
| 400 000 | 0 | 0 | 0.03 | 0.09 | 0.08 |
| 2 000 000 | 0 | 0 | 0 | 0 | 0 |
It is necessary to mention that the pore volume accessible to a given molecule is determined by its size (weight) and the pore size distribution of packing and can be defined by the following formula:
 |
where Kav is the distribution coefficient (the factor of pore volume available for a given molecule), Ve is elution (retention) volume, Vo is void (interstitial) volume and Vt is total column volume.
| Types | Bed Volume [ml g-1 dry] | Exclusion Limit | Fractionation Range | |
|---|---|---|---|---|
| MW dextrans | MW proteins, g mol-1 | MW proteins, g mol-1 | ||
| MT 50 | 3 - 5 | 50 000 | 1 x 105 | 20 000 - 70 000 |
| MT 100 | 9 - 11 | 100 000 | 2 x 106 | 30 000 - 1 000 000 |
| MT 200 | 13 - 16 | 200 000 | 4 x 106 | 200 000 - 2 000 000 |
| MT 500 | 18 - 21 | 500 000 | 5 x 106 | 300 000 - 4 000 000 |
Other technical data
Values of pure Bead Cellulose only (non-magnetic and non-derivated)
| Temperature Resistance: | in wet state, pH 7.0, for 1 hour: 120°C |
|---|---|
| Stability within pH Range: | 1 - 14, NaOH or HCl (c = 1 mol l-1) |
| Stability in Salt Solutions: | with ionic strength up to 10 mol l-1 |
| Swelling / Shrinkage (vol %): | with changes in pH, salt concentration (over the whole range of pH anionic strength): 1 max. |
| with replacing of aqueous solutions by polar organic solvents: 10 max. | |
| Chemical Resistance: | aqueous solutions, buffer, organics, detergents and chaotropic agents (including SDS, 8 M urea) |