Iontosorb SHP

Strongly acidic cation exchanger

IONTOSORB SHP is a strongly acidic cation exchanger based on Bead Cellulose with 3-sulpho-2-hydroxypropyl- groups:

11shp.gif, 1 kB

Its total volume capacity can move in the range 0.04 to 0.15 mmol/ml, adequate mass capacity is 0.15 to 0.45 mmol/g dry matter. According to customerĀ“s demand the sorbents volume capacity can be varied in the range given by the mass capacity and water content. Water content moves in connection with the volume capacity in the range 58 to 85 %. Iontosorb SHP can be used in the chromatographic separations of proteins, peptides, amino acids, nucleic acid components and other biological substances.

Application

Fast protein liquid chromatography
Original egg white (top), eluate from IONTOSORB MG CM 100 (middle) and eluate from Iontosorb MG SHP 100 (bottom)
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Set of five overlapping spectra of a lysozyme peak
Set of five overlapping spectra of a lysozyme peak (retention time8.424 min) between 190 and 400 nm obtained after Iontosorb MG SHP isolation. The spectra were taken 8.068, 8.255, 8.415, 8.575 and 8.735 min, respectively
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Equilibrium adsorption isotherms of hen egg white lysozyme
Using Iontosorb MG CM 100 (full triangles) and Iontosorb MG SHP 100 (full squares) as adsorbents:
Ceq - equilibrium liquid-phase concentration of the unadsorbed (free) lysozyme;
qeq - equilibrium solid-phase concentration of the adsorbed lysozyme
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In: I. Safarik, Z. Sabatkova, O. Tokar, M. Safarikova: MAGNETIC CATION EXCHANGE ISOLATION OF LYSOZYME FROM NATIVE HEG EGG WHITE. Food Technol. Biotechnol., 45 (2007), p.355-359.

Whole article on-line: http://www.ftb.com.hr/45/45-355.pdf

Technical parameters

Porozity TypeParticle Size (μm)

SHP 100

30 - 50
50 - 80
80 - 100
100 - 250
250 - 500

SHP 200
SHP 500

50 - 80
80 - 100
100 - 250
250 - 500

Where to continue?

  ↑  UP THIS PAGE.
 ←  General informations.
 →  IONTOSORB CM is a weakly acidic cation exchanger.
 →  IONTOSORB P is a medium acidic cation exchanger.
 →  IONTOSORB TMAHP is a strongly basic anion exchanger.
 →  IONTOSORB DEAE is a weakly basic anion exchanger.
 →  Test Kit for Ion Exchange Chromatography for research work.