Iontosorb CM

Weakly acidic cation exchanger

A weakly acidic cation exchanger based on Bead Cellulose with carboxylic functional groups:

cm.jpg, 67 kB

The total volume capacity can be in the range 0.005 - 0.25 mmol/ml, the adequate mass capacity is 0.1 to 1.3 mmol/g dry matter. According to the customer's demands the sorbent volume capacity can be varied in the range given by the mass capacity and water content. The water content of Iontosorb CM moves in the connection with the volume capacity in the range 74 to 89 %.

Iontosorb CM is suitable for chromatographic separations of basic proteins, basic amino acids, peptides, lipids and similar substances. Beside that, it may be used for enzyme immobilization.

Applications

Trypsin and Chymotripsyn activity in pooled fractions obtained from Iontosorb CM column (pH 6.0)
Column 2 x 8 cm equilibrated with 10 mM Tris-HCl pH 6.0 buffer, containing 10 mM calcium chloride
Adsorbed fractions were elued with 1 M NaCl in starting buffer after washing-out the unadsorbed proteins.
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Source: ISOLATION AND CHARACTERISATION OF THE PROTEOLITIC ENZYMES OF CARP HEPATOPANCREAS; M. Kminkova, Z. Moucka, Jiri Kucera; Potravinarske vedy, 15 (1997) 351-362.


Semi-preparative ion exchange chromatography of alkaline proteinase on Iontosorb CM
The column 21 x 130 mm was equilibrated with phosphate buffer and eluted with linear gradients of 0-0.5 and 0.5-1 mol/L NaCl, respectively, in the above buffer; combined fractions (7-10) from theproceding IEC dialyzed against phosphate buffer were loaded; the flow rate 0.5 mL/min, 5 mL fractions were colected
N - fraction number, P - proteins (mg, circles), A620/h - proteolytic activity (squares), NaCl gradient (mol/L, dashed line).
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Source: USE OF BEAD CELLULOSE DERIVATIVES TO ISOLATION OF BACTERIAL ALKALINE PROTEINASE BY COLUMN LIQUID CHROMATOGRAPHY; P. Gemeiner, V. Spanik, A. Snajdrova, E. Stratilova, M. Horvathova, D. Hagarova and O. Markovic; Folia Microbiol. 36 (1991) 283-293.


Fast protein liquid chromatography
Original egg white (top), eluate from IONTOSORB MG CM 100 (middle) and eluate from Iontosorb MG SHP 100 (bottom)
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Electrophoretic patterns of egg white proteins and lysozyme isolated by Iontosorb MG CM 100
Lane 1- molecular mass markers;
lane 2-egg white (9.3 ug of proteins loaded);
lane 3-egg white after finishing the adsorption process (8.1 ug of proteins loaded);
lane 4-lysozyme after elution from Iontosorb MG CM 100 (8.75 ug of proteins loaded);
lane 5-commercial lysozyme (Fluka, Germanny, 12 ug of proteins loaded). Arrow indicates the lysozyme position.
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Equilibrium adsorption isotherms of hen egg white lysozyme
Equilibrium adsorption isotherms of hen egg white lysozyme using Iontosorb MG CM 100 (full triangles) and Iontosorb MG SHP 100 (full squares) as adsorbents:
Ceq - equilibrium liquid-phase concentration of the unadsorbed (free) lysozyme;
qeq - equilibrium solid-phase concentration of the adsorbed lysozyme
beadh8.gif, 8 kB

Source: I. Safarik, Z. Sabatkova, O. Tokar, M. Safarikova: MAGNETIC CATION EXCHANGE ISOLATION OF LYSOZYME FROM NATIVE HEG EGG WHITE. Food Technol. Biotechnol., 45 (2007), p.355-359.

Whole article on-line: http://www.ftb.com.hr/45/45-355.pdf

Technical parameters

Non-magnetic CM
Porozity TypeParticle Size (μm)

CM 100

30 - 50
50 - 80
80 - 100
100 - 250
250 - 500

CM 200
CM 500

50 - 80
80 - 100
100 - 250
250 - 500
Magnetic CM
Porozity TypeParticle Size (μm)

MG CM 100

30 - 50
50 - 80
80 - 100

MG CM 200

50 - 80
80 - 100

In an order a demanded content of functional groups shall be given (e.g. IONTOSORB MG CM 100M means demanded content of functional groups 0.10 - 0.20 mmol Cu/g dry). IONTOSORB MG CM (magnetic sorbents) is delivered with the following content of functional groups:

Magnetic CM
SorbentsContent of functional groups
mmol Cu / gram dry sorbents
IONTOSORB MG CM 100L low 0.05 - 0.08
IONTOSORB MG CM 200L
IONTOSORB MG CM 100M medium 0.10 - 0.20
IONTOSORB MG CM 200M
IONTOSORB MG CM 100H high 0.25 - 0.45
IONTOSORB MG CM 200H
IONTOSORB MG CM 100VH very high 0.50 - 0.65
IONTOSORB MG CM 200VH

Where to continue?

  ↑  UP THIS PAGE.
 ←  General informations.
 ←  IONTOSORB SHP is a strongly acidic cation exchanger.
 →  IONTOSORB P is a medium acidic cation exchanger.
 →  IONTOSORB TMAHP is a strongly basic anion exchanger.
 →  IONTOSORB DEAE is a weakly basic anion exchanger.
 →  Test Kit for Ion Exchange Chromatography for research work.