Iontosorb BLUE 2

is an adsorbent based on bead-form macroporous cellulose which is used for affinity chromatography (Dye Ligand Chromatography). It contains the covalently bound dye Reactive Blue 2:

brown-10.gif, 1 kB

Iontosorb BLUE 2 is a specific sorbent with affinity to wide group of enzymes. Iontosorb BLUE 2 binds also the serum albumin and can be used for the selective removal of albumin from other plasma proteins.

Applications

COMPETITIVE ELUTION OF LACTATE DEHYDROGENASE FROM CIBACRON BLUE CELLULOSE (Iontosorb BLUE-2) WITH CIBACRON BLUE-DEXTRAN

D. Mislovicova, P. Gemeiner, E. Stratilova and M. Horvathova

Journal of Chromatography, 510 (1990) 197-204

Elution profiles of LDH of beef muscle extract from Iontosorb BLUE-2
dlc02

Eluting agents: (squares) 3 M KCl

(full circle) 1mM NADH

(circle) 50 mM Cibacron Blue Dextran T 10

(full squares) equilibration 20 mM phosphate buffer (pH 8.5)

Technical parameters

Porozity TypeParticle Size (μm)

BLUE-2 100

30 - 50
50 - 80
80 - 100
100 - 250
250 - 500

BLUE-2 200
BLUE-2 500

50 - 80
80 - 100
100 - 250
250 - 500

Where to continue?

  ↑  UP THIS PAGE.
 →  Iontosorb RED is a sorbent with fixed Reactive Red 120 (Procion© Red HE3B)
 →  Iontosorb BLUE 4 is a sorbent with fixed Reactive Blue 4 (Reactive Blue MRB ) TC
 →  Iontosorb GREEN 5 is a sorbent with fixed Reactive Green 5 (Reactive Green H4G) TC
 →  Iontosorb GREEN 19 is a sorbent with fixed Reactive Green 19 (Reactive Green HE4BD) TC
 →  Iontosorb GREEN 19A is a sorbent with fixed Reactive Green 19A (Reactive Green HE4BD) TC
 →  Iontosorb YELLOW is a sorbent with fixed Reactive Yellow 86 (Reactive Yellow M8G) TC
 →  Iontosorb BROWN is a sorbent with fixed Reactive Brown 10 (Reactive Brown M4R ) TC
 →  Test Kit for Dye-Ligand Chromatography for Research Work